Investigating Gene Function in Cereal Rust Fungi by Plant-Mediated Virus-Induced Gene Silencing.
Identifieur interne : 000502 ( Main/Exploration ); précédent : 000501; suivant : 000503Investigating Gene Function in Cereal Rust Fungi by Plant-Mediated Virus-Induced Gene Silencing.
Auteurs : Vinay Panwar [Canada] ; Guus Bakkeren [Canada]Source :
- Methods in molecular biology (Clifton, N.J.) [ 1940-6029 ] ; 2017.
Descripteurs français
- KwdFr :
- Basidiomycota (génétique), Basidiomycota (virologie), Extinction de l'expression des gènes (MeSH), Grains comestibles (microbiologie), Grains comestibles (virologie), Gènes fongiques (MeSH), Génomique (méthodes), Maladies des plantes (microbiologie), Triticum (microbiologie), Triticum (virologie), Vecteurs génétiques (génétique), Virus des plantes (génétique).
- MESH :
- génétique : Basidiomycota, Vecteurs génétiques, Virus des plantes.
- microbiologie : Grains comestibles, Maladies des plantes, Triticum.
- méthodes : Génomique.
- virologie : Basidiomycota, Grains comestibles, Triticum.
- Extinction de l'expression des gènes, Gènes fongiques.
English descriptors
- KwdEn :
- Basidiomycota (genetics), Basidiomycota (virology), Edible Grain (microbiology), Edible Grain (virology), Gene Silencing (MeSH), Genes, Fungal (MeSH), Genetic Vectors (genetics), Genomics (methods), Plant Diseases (microbiology), Plant Viruses (genetics), Triticum (microbiology), Triticum (virology).
- MESH :
- genetics : Basidiomycota, Genetic Vectors, Plant Viruses.
- methods : Genomics.
- microbiology : Edible Grain, Plant Diseases, Triticum.
- virology : Basidiomycota, Edible Grain, Triticum.
- Gene Silencing, Genes, Fungal.
Abstract
Cereal rust fungi are destructive pathogens, threatening grain production worldwide. Targeted breeding for resistance utilizing host resistance genes has been effective. However, breakdown of resistance occurs frequently and continued efforts are needed to understand how these fungi overcome resistance and to expand the range of available resistance genes. Whole genome sequencing, transcriptomic and proteomic studies followed by genome-wide computational and comparative analyses have identified large repertoire of genes in rust fungi among which are candidates predicted to code for pathogenicity and virulence factors. Some of these genes represent defence triggering avirulence effectors. However, functions of most genes still needs to be assessed to understand the biology of these obligate biotrophic pathogens. Since genetic manipulations such as gene deletion and genetic transformation are not yet feasible in rust fungi, performing functional gene studies is challenging. Recently, Host-induced gene silencing (HIGS) has emerged as a useful tool to characterize gene function in rust fungi while infecting and growing in host plants. We utilized Barley stripe mosaic virus-mediated virus induced gene silencing (BSMV-VIGS) to induce HIGS of candidate rust fungal genes in the wheat host to determine their role in plant-fungal interactions. Here, we describe the methods for using BSMV-VIGS in wheat for functional genomics study in cereal rust fungi.
DOI: 10.1007/978-1-4939-7249-4_10
PubMed: 28856645
Affiliations:
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Targeted breeding for resistance utilizing host resistance genes has been effective. However, breakdown of resistance occurs frequently and continued efforts are needed to understand how these fungi overcome resistance and to expand the range of available resistance genes. Whole genome sequencing, transcriptomic and proteomic studies followed by genome-wide computational and comparative analyses have identified large repertoire of genes in rust fungi among which are candidates predicted to code for pathogenicity and virulence factors. Some of these genes represent defence triggering avirulence effectors. However, functions of most genes still needs to be assessed to understand the biology of these obligate biotrophic pathogens. Since genetic manipulations such as gene deletion and genetic transformation are not yet feasible in rust fungi, performing functional gene studies is challenging. Recently, Host-induced gene silencing (HIGS) has emerged as a useful tool to characterize gene function in rust fungi while infecting and growing in host plants. We utilized Barley stripe mosaic virus-mediated virus induced gene silencing (BSMV-VIGS) to induce HIGS of candidate rust fungal genes in the wheat host to determine their role in plant-fungal interactions. Here, we describe the methods for using BSMV-VIGS in wheat for functional genomics study in cereal rust fungi.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">28856645</PMID><DateCompleted><Year>2018</Year><Month>05</Month><Day>07</Day></DateCompleted><DateRevised><Year>2018</Year><Month>08</Month><Day>09</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1940-6029</ISSN><JournalIssue CitedMedium="Internet"><Volume>1659</Volume><PubDate><Year>2017</Year></PubDate></JournalIssue><Title>Methods in molecular biology (Clifton, N.J.)</Title><ISOAbbreviation>Methods Mol Biol</ISOAbbreviation></Journal><ArticleTitle>Investigating Gene Function in Cereal Rust Fungi by Plant-Mediated Virus-Induced Gene Silencing.</ArticleTitle><Pagination><MedlinePgn>115-124</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/978-1-4939-7249-4_10</ELocationID><Abstract><AbstractText>Cereal rust fungi are destructive pathogens, threatening grain production worldwide. Targeted breeding for resistance utilizing host resistance genes has been effective. However, breakdown of resistance occurs frequently and continued efforts are needed to understand how these fungi overcome resistance and to expand the range of available resistance genes. Whole genome sequencing, transcriptomic and proteomic studies followed by genome-wide computational and comparative analyses have identified large repertoire of genes in rust fungi among which are candidates predicted to code for pathogenicity and virulence factors. Some of these genes represent defence triggering avirulence effectors. However, functions of most genes still needs to be assessed to understand the biology of these obligate biotrophic pathogens. Since genetic manipulations such as gene deletion and genetic transformation are not yet feasible in rust fungi, performing functional gene studies is challenging. Recently, Host-induced gene silencing (HIGS) has emerged as a useful tool to characterize gene function in rust fungi while infecting and growing in host plants. We utilized Barley stripe mosaic virus-mediated virus induced gene silencing (BSMV-VIGS) to induce HIGS of candidate rust fungal genes in the wheat host to determine their role in plant-fungal interactions. Here, we describe the methods for using BSMV-VIGS in wheat for functional genomics study in cereal rust fungi.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Panwar</LastName><ForeName>Vinay</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>Morden Research and Development Centre, Agriculture and Agri-Food Canada, 101 Route 100, Unit 100, Morden, MB, Canada. Gene_Silencing@yahoo.com.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>National Research Council Canada, Plant Biotechnology Institute, Saskatoon, SK, Canada. 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